Journal: Frontiers in Cellular Neuroscience

Article Title: Human Mesenchymal Stem Cells Prevent Neurological Complications of Radiotherapy

doi: 10.3389/fncel.2019.00204

Figure Lengend Snippet: hMSC administration does not compromise survival of glioma-bearing mice. (A) Schematic representation of the study design. U87 glioma cells were intracranially transplanted into the striatum of nude mice. After 10 days, mice received whole-brain radiation (total dose of 10 Gy) and the day after, 5 × 10 5 hMSCs were administered intranasally once per week for 4 weeks and time of death was monitored. (B) Xenolight DiR-labeled U87 glioma cells were locally transplanted into the striatum of nude mice. Images show bioluminescence activity in a representative animal 24 h after cell transplantation. (C) Animal weight was measured during the course of the experiment. Note the weight loss after radiation exposure. (D) Kaplan–Meier curve showing the percentage of survival of glioma-bearing mice. Note that IR and IR+MSC mice exhibited similar response, increasing survival as compared to NonIR mice. (E) Histological images of brain tumors at the time of death (indicated as days post tumor implantation, PTI) in the last individual surviving for each experimental group. H&E: Hematoxylin and eosin staining. Scale bar 1 mm (25 μm for details). Data are represented as mean ± SEM. n = 11–12 per group. ∗ p < 0.0001 compared to U87 NonIR mice; Multiple t -test (C) , Log-rank test (D) .

Article Snippet: For evaluation of cell biodistribution, cultured hMSCs were incubated with 400 μg/mL XenoLight DiR fluorescent dye (Perkin Elmer, Inc., Boston, MA) for 30 min at 37°C before transplantation.

Techniques: Labeling, Activity Assay, Transplantation Assay, Tumor Implantation, Staining

Journal: Science advances

Article Title: Endogenous/exogenous dual-responsive nanozyme for photothermally enhanced ferroptosis-immune reciprocal synergistic tumor therapy.

doi: 10.1126/sciadv.adq3870

Figure Lengend Snippet: Fig. 4. Cellular uptake and in vitro therapeutic effect. (A) Fluorescence images of 4T1 cells incubated with RhB-MCMSFT for various time intervals. h, hours. (B) Fluores- cence images of caspase-3 (Cas-3) immunofluorescence staining and LPO staining in 4T1 cells after incubation with PBS or MCMSFT. (C) The cell viability of 4T1 cells treated with MCMSFT in the presence of DFO (100 μM), Fer-1(10 μM), or DEVD (50 μM). (D) The cell viability and (E) live/dead cell staining images of 4T1 cells treated with different formulations with or without laser irradiation. The data are presented as the means ± SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001. NS, no significance difference. AM, acetoxymethyl ester.

Article Snippet: Near- infrared fluorescence dye DiR was purchased from Abbkine Scientific (Wuhan, China).

Techniques: In Vitro, Fluorescence, Incubation, Immunofluorescence, Staining, Irradiation

Journal: Science advances

Article Title: Endogenous/exogenous dual-responsive nanozyme for photothermally enhanced ferroptosis-immune reciprocal synergistic tumor therapy.

doi: 10.1126/sciadv.adq3870

Figure Lengend Snippet: Fig. 5. Potential mechanism of hybrid multiple cell deaths induction. (A) The proposed molecular mechanisms of MCMSFT-induced hybrid cell death including apop- tosis, ferroptosis, and necroptosis. (B) Representative Western blot images of GPX4, ferritin, Cas-3, and PD-L1 expression measurement after different treatments. GAPDH, glyceraldehyde-3-phosphate dehydrogenase. (C) Bio-TEM images of 4T1 cells treated with PBS or MCMSFT. (D to G) Fluorescence staining images of intracellular Fe2+, ROS, GSH, and LPO detection in 4T1 cells after different treatments.

Article Snippet: Near- infrared fluorescence dye DiR was purchased from Abbkine Scientific (Wuhan, China).

Techniques: Western Blot, Expressing, Fluorescence, Staining

Journal: Science advances

Article Title: Endogenous/exogenous dual-responsive nanozyme for photothermally enhanced ferroptosis-immune reciprocal synergistic tumor therapy.

doi: 10.1126/sciadv.adq3870

Figure Lengend Snippet: Fig. 7. In vivo live imaging and therapeutic efficacy. (A) Fluorescence images of 4T1 tumor–bearing mice at different time points after intravenous administration of free DiR or DiR-CMSFT. (B) Time-dependent photothermal images of 4T1 tumor–bearing mice after intravenous administration of saline or MCMSFT with laser irradiation. (C) Schematic illustration of 4T1 subcutaneous tumor–bearing mouse model establishment and treatment schedule. (D) Photograph of tumors from mice after various treatments. (E) Representative hematoxylin and eosin (H&E), terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling (TUNEL), and Ki67 staining images of tumor sections. Scale bars, 100 μm. (F) Representative LPO, CD8, and Cas-3 staining images of tumor sections. Scale bars, 100 μm.

Article Snippet: Near- infrared fluorescence dye DiR was purchased from Abbkine Scientific (Wuhan, China).

Techniques: In Vivo, Imaging, Drug discovery, Fluorescence, Saline, Irradiation, End Labeling, TUNEL Assay, Staining

Journal: Science advances

Article Title: Endogenous/exogenous dual-responsive nanozyme for photothermally enhanced ferroptosis-immune reciprocal synergistic tumor therapy.

doi: 10.1126/sciadv.adq3870

Figure Lengend Snippet: Fig. 8. Ferroptosis-immune reciprocal effect and immune antitumor activity. (A) Representative fluorescence and immunofluorescence staining images of ROS, GPX4, and ferritin. Scale bars, 100 μm. (B) Representative immunofluorescence staining images of CRT and PD-L1. Scale bars, 100 μm. (C) ELISA measurement of IFN-γ levels in serum collected from mice after various treatments. (D) Representative immunofluorescence staining images of SLC7A11 and SLC3A2. Scale bars, 100 μm. (E) Representative flow cytometry plots and (F) quantitative analysis of mature DCs (CD11C+CD80+CD86+) in tumor-draining lymph nodes (LNs). (G to L) Representative flow cytometry plots and quantitative analysis of cytotoxic T lymphocytes (CD3+CD8+) and helper T lymphocytes (CD3+CD4+) in the spleen and tumor. The data are pre- sented as the means ± SD, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Article Snippet: Near- infrared fluorescence dye DiR was purchased from Abbkine Scientific (Wuhan, China).

Techniques: Activity Assay, Fluorescence, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay, Flow Cytometry

Journal: Science advances

Article Title: Endogenous/exogenous dual-responsive nanozyme for photothermally enhanced ferroptosis-immune reciprocal synergistic tumor therapy.

doi: 10.1126/sciadv.adq3870

Figure Lengend Snippet: Fig. 9. Suppression of lung metastasis and rechallenge tumor. (A) Schematic illustration of lung metastasis tumor–bearing mouse model establishment and treatment schedule. (B and C) Photographs of Bouin’s solution–stained lung tissues and quantitative analysis of metastasis nodules with different treatments. (D) H&E and immuno- fluorescence staining images of CD8 and Ki67 in the lung sections with different treatments. (E) Schematic illustration of rechallenge tumor–bearing mouse model estab- lishment and treatment schedule. (F) Observation of primary tumors and rechallenge tumors growth with different treatments. (G) Quantitative analysis of the rechallenge tumor growth of mice with different treatments. (H) Immunofluorescence staining images of CD8 and Ki67 in the tumor sections with different treatments. (I) Survival curve of mice with different treatments. The data are presented as the means ± SD, n = 5, **P < 0.01 and ***P < 0.001.

Article Snippet: Near- infrared fluorescence dye DiR was purchased from Abbkine Scientific (Wuhan, China).

Techniques: Staining, Fluorescence, Immunofluorescence