Journal: Frontiers in Cellular Neuroscience

Article Title: Human Mesenchymal Stem Cells Prevent Neurological Complications of Radiotherapy

doi: 10.3389/fncel.2019.00204

Figure Lengend Snippet: hMSC administration does not compromise survival of glioma-bearing mice. (A) Schematic representation of the study design. U87 glioma cells were intracranially transplanted into the striatum of nude mice. After 10 days, mice received whole-brain radiation (total dose of 10 Gy) and the day after, 5 × 10 5 hMSCs were administered intranasally once per week for 4 weeks and time of death was monitored. (B) Xenolight DiR-labeled U87 glioma cells were locally transplanted into the striatum of nude mice. Images show bioluminescence activity in a representative animal 24 h after cell transplantation. (C) Animal weight was measured during the course of the experiment. Note the weight loss after radiation exposure. (D) Kaplan–Meier curve showing the percentage of survival of glioma-bearing mice. Note that IR and IR+MSC mice exhibited similar response, increasing survival as compared to NonIR mice. (E) Histological images of brain tumors at the time of death (indicated as days post tumor implantation, PTI) in the last individual surviving for each experimental group. H&E: Hematoxylin and eosin staining. Scale bar 1 mm (25 μm for details). Data are represented as mean ± SEM. n = 11–12 per group. ∗ p < 0.0001 compared to U87 NonIR mice; Multiple t -test (C) , Log-rank test (D) .

Article Snippet: For evaluation of cell biodistribution, cultured hMSCs were incubated with 400 μg/mL XenoLight DiR fluorescent dye (Perkin Elmer, Inc., Boston, MA) for 30 min at 37°C before transplantation.

Techniques: Labeling, Activity Assay, Transplantation Assay, Tumor Implantation, Staining

Journal: Cellular and Molecular Immunology

Article Title: Kinetics of the accumulation of group 2 innate lymphoid cells in IL-33-induced and IL-25-induced murine models of asthma: a potential role for the chemokine CXCL16

doi: 10.1038/s41423-018-0182-0

Figure Lengend Snippet: Mice were injected with XenoLight DiR-stained ILC2s intravenously, then intranasally challenged with IL-25, IL-33, CXCL16 or CCL25 (all 100 nmoL/L) and imaged ventrally with an IVIS Spectrum machine at 0, 6, 24 and 30 h. Thymus, Med LN, lungs, heart, liver, spleen, kidney and bone were harvested for ex vivo imaging at the 30-h time point. *p < 0.05 (n = 3 for each group)

Article Snippet: ILC2s isolated from the lung tissues of mice nasally challenged with rmIL-33 as described above were incubated with 320 μg/ml XenoLight DiR fluorescent dye (Caliper Life Sciences, Inc., Hopkinton MA, USA).

Techniques: Injection, Staining, Ex Vivo, Imaging